Journal: Cells
Article Title: Interferon-Gamma Primed Human Clonal Mesenchymal Stromal Cell Sheets Exhibit Enhanced Immunosuppressive Function
doi: 10.3390/cells11233738
Figure Lengend Snippet: Duration of IFN-γ priming is directly related to enhanced hcBMSC sheet gene expression of immunomodulatory factors. Quantitative real-time PCR gene expression for immunomodulatory genes ( a ) human leukocyte antigen DR (HLA-DRA), ( b ) programmed death ligand-1 (PD-L1), ( c ) indoleamine 2,3-dioxygenase (IDO-1), ( d ) interleukin 10 (IL-10), and ( e ) prostaglandin E synthase 2 (PTGES2). All gene expression is normalized to GAPDH, fold change is relative to non-primed control hBMSC sheet sample. Error bars represent means ± SE ( n = 6) (n.s. p ≥ 0.05, ** p < 0.01, *** p < 0.001) Statistical analysis p values are compared to non-IFN-γ hcBMSC sheets (non-IFN-γ, control).
Article Snippet: The concentration of soluble IL-10, IDO, PGE2, and PD-L1 per sample was quantified using human IL-10 DuoSet ELISA kit (R&D Systems, Minneapolis, MN, USA), human indoleamine 2,3-dioxygenase/IDO DuoSet ELISA kit (R&D Systems, Minneapolis, MN, USA), prostaglandin E2 Parameter Assay Kit (R&D Systems, Minneapolis, MN, USA), and human PD-L1 DuoSet ELISA Kit (R&D Systems, Minneapolis, MN, USA), respectively.
Techniques: Gene Expression, Real-time Polymerase Chain Reaction, Control